Blood, 1960, Vol. 16, No. 4, pp. 1424-1432.
© 1960 American Society of Hematology, Inc.
A Cooperative Assay of a Sample with Erythropoietic
Stimulating Activity
GEOFFREY KEIGHLEY ,
PETER H. LOWY ,
HENRY BORSOOK ,
EUGENE GOLDWASSER ,
ALBERT S. GORDON ,
THEODORE C. PRENTICE ,
WALTER A. RAMBACH ,
FREDERICK STOHLMAN JR. , and
DONALD C. VAN DYKE
A batch of erythropoietically active material made from the plasma of
phenylhydrazine treated rabbits was distributed to seven laboratories in this
country for the purpose of comparing assay methods. The results of the
assays using 10 different methods or major variations are presented.
Most of the assay methods being employed were represented so that other
laboratories should be able to compare their results to those obtained in
this study.
The importance of obtaining some uniform basis for comparison of activity
of erythrocyte stimulating factor between laboratories has been emphasized
and a tentative unit has been proposed based on a net incorporation into rat
red cells of 20 per cent of injected Fe59, using a standardized assay procedure.
Submitted on March 14, 1960
Accepted on June 28, 1960