Blood, 1960, Vol. 16, No. 3, pp. 1330-1337.
© 1960 American Society of Hematology, Inc.
Studies in Experimental Eosinophilia. II. Induction
of Peritoneal Eosinophilia by the Transfer
of Tissues and Tissue Extracts
MORTIMER LITT 1
1 Department of Bacteriology and Immunology, Harvard Medical School, Boston,
Mass.
Tissues taken from one guinea pig were transferred into the peritoneal
cavity of another, and the total number of peritoneal eosinophils in the recipient was determined 24 hours after transfer. Peritoneal eosinophilia resulted
in the recipients when peritoneal lining, lungs, blood or serum were taken
from an animal previously prepared by repeated injections of foreign protein
and transferred along with some of the same protein. With lung, but not with
peritoneal lining, some eosinophil responses were noted when the protein used
to prepare the donor was omitted, and when either the donor or recipient
was a normal animal.
Extracts of peritoneal lining or lung also induced peritoneal eosinophilia.
Isolated instances of activity occurred in extracts of normal tissues, but most
of the positive responses were induced when extracts from animals previously
prepared with foreign protein were accompanied by the same protein.
Eosinophilotactic activity remained inside dialysis bags. It was stable to
56 C. for 
hour, 4 C. for 24 hours, and -5 C. for two days. The activity disappeared with the removal of trichloracetic acid-precipitable material.
These experiments support the view that eosinophilia may occur in the absence of actively acquired antibody, and that an eosinophilotactic mechanism
may be passively transferred from one guinea pig to another.
Submitted on January 16, 1960
Accepted on April 2, 1960
