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Blood, 1960, Vol. 16, No. 2, pp. 1122-1132.
© 1960 American Society of Hematology, Inc.

Radioautographic Investigations on DNA and Protein Metabolism in 2 Cases of Di Guglielmo’s Disease

FELICE GAVOSTO 1, GIOVANNI MARAINI 1, and ALESSANDRO PILERI 1

1 Medical Clinic of Turin Medical School (Director, Prof. G. C. Dogliotti) Turin, Italy.

H3-thymidine and H3-DL-leucine incorporation in the erythroblasts of the bone marrow from a case of acute and one of chronic Di Guglielmo’s disease was studied. A similar investigation was also carried out with bone marrow from 4 normal subjects. The high resolution of the radioautographic technic employed allowed a comparative study at the cellular and subcellular level.

A definitely lowered thymidine uptake was observed in the cells of acute erythremic myelosis, while increased incorporation of DL-leucine was detected in both the acute and the chronic form of this disease.

Submitted on August 4, 1959
Accepted on February 18, 1960


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