Blood, 1960, Vol. 16, No. 1, pp. 1012-1019.
© 1960 American Society of Hematology, Inc.
The Morphology of Buffy Coat in Normal Human Adults
P. EFRATI 1 and
L. ROZENSZAJN 1
1 Laboratory for Blood Morphology, The Kaplan Hospital of the Labourer’s
Sick Fund, Rehovoth, Israel.
The technic of preparation of smears from buffy coats was described.
Fifty-five samples of buffy coat from healthy adults were examined.
Twenty-nine were from men, 26 from women.
In all cases nuclear fragments of megakaryocytes were found, on the
average 21.8 nuclear fragments per 1 ml. of blood.
In all cases atypical mononuclear cells, 10.2/2000 mononuclear cells, were
found.
In 42 of 52 examined subjects metamyelocytes and/or myelocytes were
found, 3.65/3000 granulocytes.
Metamyelocytes, myelocytes and nuclear fragments of megakaryocytes
were more commonly found in males than in females.
So-called atypical mononuclear cells were found in small number in all
the subjects. Their possible pathologic significance was discussed. It was
suggested that these cells were probably normal though rare elements of
peripheral blood.
The advantage of this method in various pathologic states was emphasized.
Submitted on September 8, 1959
Accepted on December 15, 1959
