Blood, 1960, Vol. 15, No. 6, pp. 909-917.
© 1960 American Society of Hematology, Inc.
Brief Note: The Use of a Special Preservation Medium for the
Maintenance of Platelet Viability at 4 C
MARIO BALDINI 1,
SHIRLEY EBBE 1,
WILLIAM DAMESHEK 1, and
Janet Donovan 1
1 Blood Research Laboratory, a unit of the Ziskind Laboratories, New England
Center Hospital, Boston, Mass., and the Department of Medicine, Tufts University School
of Medicine.
Viability of rabbit platelets stored for 1 to 6 days in different media at 4 C.
was studied using the Cr51-platelet survival technic.
Platelets stored for 24 hours in Triton-saline or DAS-gelatin had practically
no viability.
When platelets were stored for 24 hours in diluted plasma, significant degrees of viability were found, but this decreased rapidly in the successive 48
hours.
The viability of platelets stored in diluted plasma containing inosine, adenine,
glucose and phosphate buffer was maintained at significant levels over a six
day period.
The data presented here suggest that viability and, consequently, the hemostatic properties of platelets stored at 4 C. can be maintained over relatively
long periods of time provided that loss of the ATP reserve of the cells can be
prevented.
Submitted on January 26, 1960
Accepted on March 11, 1960
