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Blood, 1960, Vol. 15, No. 5, pp. 637-645.
© 1960 American Society of Hematology, Inc.

A Simple Method for the Assay of Factor VIII

LUIS JOSÉ BERGNA 1

1 Instituto de Investigaciones Hematológicas (Director Dr. A. Pavlovsky) de la Academia Nacional de Medicina, Buenos Aires, Argentina.

A simple method for the assay of factor VIII activity, based on the thromboplastin generation test of Biggs and Douglas, has been described.

Bovine serum is used as a source of factor V and serum factors, and plasma from a hemophiliac is not required. The concentration of factor VIII of the test plasma is compared to that of a standard normal human plasma. The results obtained with the method have been fairly reproducible.


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