Blood, 1960, Vol. 15, No. 4, pp. 525-533.
© 1960 American Society of Hematology, Inc.
The Effects of Concentrated Eluted Anti-Red Blood Cell
Antibodies on the in Vivo Survival of Normal
Red Blood Cells
NEIL W. CULP 1 and
HUGH CHAPLIN JR. 1
1 Division of Hematology, Departments of Medicine and Preventive Medicine,
Washington University School of Medicine and the Barnes Hospital, St. Louis, Mo.
1. A method has been described for the preparation and sterilization of a
concentrated eluate from human red cell stroma.
2. Red cells sensitized by such an eluate prepared from normal control
red cells showed entirely normal in vivo survival, as did cells sensitized by
eluate from anti-H coated cells.
3. Sensitization of red cells by concentrated eluates from a patient with
Coombs-negative acquired hemolytic anemia and from a patient with Coombs-positive acquired hemolytic anemia did not cause significant alteration in the
in vivo survival of the red cells.
4. Red cells sensitized by the concentrated eluate from anti-D sensitized
cells disappeared from the recipient’s circulation very rapidly and were sequestered in the spleen, indicating preservation of the physiologic properties
of the antibody throughout the elution, concentration and sterilization procedures.
Submitted on June 22, 1959
Accepted on September 27, 1959
