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Blood, 1960, Vol. 15, No. 1, pp. 45-51.
© 1960 American Society of Hematology, Inc.

Platelet Life Span in Normal, Splenectomized and Hypersplenic Rats

PETER F. HJORT 1 and HELEN PAPUTCHIS 1

1 Department of Medicine, University of Washington School of Medicine, Seattle, Wash.

1. A method is described for the isolation of rat platelets. The method gives high yields of platelets with negligible white and red cell contamination.

2. Rat platelets were labeled in vivo with radioactive diisopropylfluorophosphate (DFP32). The platelet life span was the same in normal, splenectomized and hypersplenic rats.

3. Some problems involved in the use of DFP32 as a blood cell label are discussed.

Submitted on April 27, 1959
Accepted on June 28, 1959


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