Blood, 1959, Vol. 14, No. 8, pp. 920-930.
© 1959 American Society of Hematology, Inc.
Detection of Leukocyte Antibodies by the
Complement Consumption Test
V. CHUDOMEL 1,
Z. JEZKOVA 1, and
J. LIBANSKY 1
1 Institute of Haematology and Blood Transfusion, Prague, Czechoslovakia.
A new method for the demonstration of leukocyte antibodies by the complement consumption test is described. For this test active serum and the
complement of the examined serum itself is used. In the presence of antibodies against the antigen used, complement consumption takes place during
the antigen-antibody reaction. This consumption can be estimated while
titrating C' by the difference between the first completely hemolyzed test
tube in the lower row (serum to which no antigen had been added), and
the test tube with complete hemolysis in the upper row (serum with added
antigen).
Two hundred-eighty normal sera, 105 sera of patients suffering from variouts internal diseases and 78 sera from patients with disorders of the blood
were examined. The complement consumption test gave positive results
more often than did the leukoagglutination method.
The method described was also successful in the demonstration of thrombocyte antibodies. We believe that it is equally suited for the detection of
various tissue antibodies.
Submitted on April 15, 1958
Accepted on November 18, 1958
