Blood, 1959, Vol. 14, No. 7, pp. 803-815.
© 1959 American Society of Hematology, Inc.
Long-Term Mixed Cultures of Human Hemic Cells, with
Granulocytic, Lymphocytic, Plasmocytic and
Erythrocytic Series Represented
JOHN H. BROOKE 1 and
EDWIN E. OSGOOD 1
1 Division of Experimental Medicine, University of Oregon Medical School,
Portland, Ore.
New culture strains containing humman hemic cells of monocytic, lymphocytic, granulocytic, plasmocytic and erythrocytic series are described with
suggestions as to maintenance of the desired cell series in culture.
The addition of cells from lymphocytic, plasmocytic and erythrocytic series
to a previously well established culture has made possible long-term culture of
these cell types, since this procedure aids in maintaining the necessary high
gradient factor for their growth and multiplication.
Although the life span of these cells has been considerably shortened in
culture and immature forms predominate, the cells show recognizable characteristics of the cell series of origin. A high population density must be
maintained in cultivation of the mixed cultures or the desired cell type may
be lost.
Submitted on September 2, 1958
Accepted on November 18, 1958
