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Blood, 1959, Vol. 14, No. 2, pp. 140-147.
© 1959 American Society of Hematology, Inc.

The Collection, Storage and Preparation of Viable Cadaver Marrow for Intravenous Use

JOSEPH W. FERREBEE 1, LEONARD ATKINS 1, HARRY L. LOCHTE JR. 1, ROBERT B. MCFARLAND 1, A. RICHARDSON JONES 1, GUSTAVE J. DAMMIN 1, and E. DONNALL THOMAS 1

1 Department of Pathology, Massachusetts General Hospital, Boston; Departments of Pathology and Pediatrics, Harvard Medical School; the Mary Imogene Bassett Hospital (affiliated with Columbia University), Cooperstown; and the Children’s Cancer Research Foundation and the Children’s Medical Center, Boston.

A procedure for collecting and storing viable cadaver marrow in a form suitable for intravenous use is described. Vertebral bone marrow is removed under sterile conditions within a few hours after death. The marrow cells are screened and suspended after centrifugation in an equal volume of a liquid medium consisting of 30 per cent glycerol and 70 per cent human serum and frozen to -80 C. in plastic bags. Suggestions for thawing, deglycerolizing and administering the cells intravenously are given.

Submitted on June 6, 1958
Accepted on July 22, 1958


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