Blood, 1959, Vol. 14, No. 11, pp. 1244-1249.
© 1959 American Society of Hematology, Inc.
Hematologic Changes in Rat Following Administration of
Desoxypyridoxine, Aminopterin, Oxythiamine and
6,7-Dimethyl-9-(2'-acetoxyethyl)isoalloxazine
V. M. DOCTOR 1
1 Section of Experimental Medicine, The University of Texas M. D. Anderson
Hospital and Tumor Institute, Houston, Tex.
1. Administration of aminopterin to rats at a level of 100 
/Kg./day for a
10 day period produced leukopenia which was rapid in onset and led quickly
to death, whereas the leukopenia produced by the administration of desoxypyridoxine at the level of 40 mg./Kg./day for a 2 week period or at 100
mg./Kg./day for a 4 week period was not accompanied by toxic effects such
as loss of appetite, diarrhea and hemorrhage and was characterized by lymphocytopenia and granulocytosis.
2. The results of combining the administration of aminopterin (70 /Kg./
day) with desoxypyridoxine (40 mg./Kg./day) for a 2 week period indicated
an additive effect of the two in the production of lymphocytopenia; the
granulocytosis resulting from desoxypyridoxine administration was counter-acted by the granulocytopenic effect of aminopterin. Also, the leukopenia
produced by the combined administration of aminopterin and desoxypyridoxine was not accompanied by the toxic effects which follow aminopterin
administration.
3. Oxythiamine alone or in combination with desoxypyridoxine or aminopterin did not influence the leukocyte count.
4. Administration of aminopterin with 6, 7-dimethyl-9-(2'-acetoxyethyl)isoalloxazine (U-2112) produced marked lymphocytopenia and granulocytopenia accompanied by toxic effects such as loss of appetite, diarrhea and hemorrhage.
See PDF for Table
Submitted on January 31, 1959
Accepted on March 9, 1958
