Blood, 1959, Vol. 14, No. 11, pp. 1213-1222.
© 1959 American Society of Hematology, Inc.
Correlation of Radioactive Hemin Formation with
Morphologic Alterations in Cultures of
Human Bone Marrow
EDWARD R. POWSNER 1 and
LAWRENCE BERMAN 1
1 Veterans Administration Hospital, Dearborn, Mich. and the Department of
Pathology, Wayne State University College of Medicine, Detroit, Mich.
A method for measurement of radioactive hemin formation has been applied to the in vitro system for culture of human erythroblasts previously
described. The results of these measurements have been shown to correlate
with a morphologic parameter of maturation, the increase of orthochromic
cells, in the same in vitro cell culture system.
The significance of these findings as well as the differences in the times
of formation of heme and the increase of orthochromic cells has been discussed.
It is proposed that both the formation of radioactive hemin and the increase of orthochromic cells may be useful in the measurement of different
aspects of the effects of various plasmas on the maturation of normal and
abnormal human erythroblasts.
Submitted on December 17, 1958
Accepted on February 21, 1959
