Blood, 1959, Vol. 14, No. 10, pp. 1103-1111.
© 1959 American Society of Hematology, Inc.
Abnormal Human Hemoglobins. I. Biosynthesis in Vitro of
Fe59-Labeled Human Hemoglobin A and Hemoglobin S
LORRAINE M. KRAUS 1,
DEMPSIE B. MORRISON 1, and
ALFRED P. KRAUS 1
1 Division of Chemistry, School of Biological Sciences and Department of
Medical Laboratories, College of Medicine, University of Tennessee and City of Memphis
Hospitals, Memphis, Tenn.
The synthesis of Fe59 labeled-hemoglobin during incubation of bone marrow from normal and sickle cell anemia (crisis and non-crisis) patients has
been investigated. The in vitro synthesis of hemoglobin S in the steady
state (noncrisis) sickle cell anemia individual was found to proceed at
a much faster rate than the synthesis of hemoglobin A from normal individuals. In the crisis of sickle cell anemia, the uptake of Fe59 and synthesis of
hemogobin S was approximately at the same level as synthesis of hemoglobin A from the normal individual. The results of this study indicate
that one of the mechanisms involved in sickle cell anemia crisis is a decrease in synthesis of hemoglobin S as compared to the synthesis of hemoglobin S during the steady state of noncrisis.
Submitted on November 20, 1958
Accepted on March 10, 1959
