Blood, 1959, Vol. 14, No. 1, pp. 50-59.
© 1959 American Society of Hematology, Inc.
Rate of Production of P32-Labeled Lymphocytes
SEYMOUR PERRY 1,
CHARLES G. CRADDOCK JR. 1,
LUTZ VENTZKE 1,
GAETANO CREPALDI 1, and
JOHN S. LAWRENCE 1
1 Department of Medicine, School of Medicine, University of California Medical
Center and Wadsworth Veterans Administration Center, Los Angeles, Calif.
1. Lymphocytes in dogs have been labeled by means of the DNA-P32 incorporation technic.
2. In the thoracic duct, a high level of lymphocyte DNA-P32 specific activity
is reached within two hours. After 24 to 30 hours, the activity falls to a
lower level which is then maintained for at least 160 hours.
3. Lymphocyte-granulocyte separations on the blood revealed that DNA-P32
specific activity reached a peak on the fourth day for both lymphocytes and
granulocytes.
4. These findings are consistent with a short maturation time for the lymphocyte and a short intravascular time.
Submitted on February 17, 1958
Accepted on April 12, 1958