Blood, 1958, Vol. 13, No. 6, pp. 543-551.
© 1958 American Society of Hematology, Inc.
Starch Block Electrophoretic Studies of Human
Hemoglobin Solutions
II. Results in Cord Blood, Thalassemia and other
Hematologic Disorders: Comparison with
Tiselius Electrophoresis
AARON M. JOSEPHSON 1,
M. S. MASRI 1,
LILY SINGER 1,
DAVID DWORKIN 1, and
KARL SINGER 1
1 Department of Hematologic Research, Medical Research Institute, Michael
Reese Hospital, Chicago, Ill.
The results of starch block electrophoresis of normal adult hemoglobin
in the various disease states has been reported. The slow-moving fraction,
A2, is elevated in all patients so far studied with thalassemia. Some patients
with Addisonian pernicious anemia also show an increase in this fraction.
Cord blood contains very little A2.
The A2 fraction has no correlation with the severity of the disease.
The A2 fraction found on starch seems to be identical with the small,
fast-moving component noted in the Tiselius apparatus at acid pH.
Iron deficiency anemia of the acquired type tends to have a lower amount
of A2 than normal, with an increase to average normal values after therapy.
Submitted on September 9, 1957
Accepted on December 15, 1957
