Blood, 1958, Vol. 13, No. 6, pp. 533-542.
© 1958 American Society of Hematology, Inc.
Starch Block Electrophoretic Studies of Human
Hemoglobin Solutions
I. Technic and Results in the Normal Adult
M. S. MASRI 1,
AARON M. JOSEPHSON 1, and
KARL SINGER 1
1 Department of Hematologic Research, Medical Research Institute, Michael
Reese Hospital, Chicago, Ill.
1. Erythrocyte hemolysates from hematologically normal, adult individuals were studied by means of starch block electrophoresis.
2. The electrophoretic patterns obtained indicated the presence, in addition to the main component of A hemoglobin A1, of three other hemoglobin
fractions designated as A2, A3 and A4, A2 and A4 move slower, while A3
moves faster in alkaline buffer. These components were isolated by elution
from the starch after separation by electrophoresis.
3. The concentrations of the various fractions in the hemolysates were
estimated from a sample of normal individuals investigated.
4. In our experiments, electrophoretic patterns obtained on starch that
has been re-used as a supportive medium for electrophoresis were somewhat
different but comparable to those obtained on starch that was used for the
first time as a supportive medium; these differences are discussed.
5. Some characterizations of the isolated components are made.
Submitted on September 7, 1957
Accepted on December 15, 1957
