Blood, 1958, Vol. 13, No. 12, pp. 1192-1197.
© 1958 American Society of Hematology, Inc.
A Method for Obtaining Polymorphonuclear Leukocytes
from Intraperitoneal Exudates
FRANCES L. ESTES 1,
SHIRLEY SMITH 1, and
JOSEPH H. GAST 1
1 Department of Biochemistry, Baylor University College of Medicine, Houston,
Texas.
By modification of existing methods, technics have been developed for obtaining polymorphonuclear leukocytes in good yield from intraperitoneal
exudates of guinea pigs. The cells were 80-98 per cent viable at the time of
harvest and they maintain this viability for at least 24 hours if stored under
refrigeration. Good yield, viability and metabolic activity are dependent on
careful handling of the cells and the exclusion of extraneous material from
the suspending medium. Since the cells are maintained without extraneous
material, they are especially suitable for metabolic studies.
Submitted on February 7, 1958
Accepted on April 20, 1958
