Blood, 1958, Vol. 13, No. 12, pp. 1149-1167.
© 1958 American Society of Hematology, Inc.
The Detroit Strains of Human Epithelial-like Cells
from Nonleukemic Peripheral Blood
LAWRENCE BERMAN 1 and
CYRIL S. STULBERG 1
1 Department of Pathology, Wayne State University College of Medicine, and
The Child Research Center of Michigan, Detroit.
1. The development of 6 strains of Ep-L cells from human blood was reported. In each instance the Ep-L cells appeared in cultures which had some
connection with the administration of estrogenic materials, either to the donor
of the blood or to the primary explant of blood in vitro. The possible role of
estrogens was discussed, but no final conclusions were reached.
2. The development of stable human Ep-L cells did not require the participation of cells from leukemic blood.
3. Morphologic, karyotypic, and mitotic data on the Detroit blood cell
strains were presented.
4. The morphogenesis of the Ep-L cells was described. The most likely
source of the Ep-L cells in blood cultures appeared to be lymphocytes, but
the role of monocytes could not be ruled out.
5. The significance of our findings with respect to transplantation studies
was indicated.
Submitted on February 28, 1958
Accepted on April 12, 1958
