Blood, 1957, Vol. 12, No. 9, pp. 822-828.
© 1957 American Society of Hematology, Inc.
In Vitro Incorporation of C14—DL-Leucine into Normal
and Leukemic White Cells
W. H. BAKER 1,
P. C. ZAMECNIK 1, and
M. L. STEPHENSON 1
1 John Collins Warren Laboratories of the Huntington Memorial Hospital of
Harvard University at the Massachusetts General Hospital, Boston, Massachusetts.
Normal white blood cells and cells from patients with leukemia are able to
incorporate C14-DL-leucine into proteins in vitro. Cells from individuals with
chronic myelogenous leukemia were able to incorporate significantly greater
amounts of the amino acid, and they were able to maintain this incorporation for
a longer period of time than the control cells.
Both types of cells had the same in vitro survival time of four days, following
storage at 3 C.
The incorporation was partially decreased in the presence of dinitrophenol, as
well as in an atmosphere of nitrogen.
Submitted on February 1, 1957
Accepted on March 7, 1957
