Blood, 1957, Vol. 12, No. 8, pp. 694-700.
© 1957 American Society of Hematology, Inc.
Studies in Leukemia
VII. The Induction of Leukemia in Swiss Mice by Means of
Cell-free Filtrates of Leukemic Mouse Brain
HAROLD M. SCHOOLMAN 1,
WILMA SPURRIER 1,
STEVEN O. SCHWARTZ 1,
PAUL B. SZANTO 1, and
LEROY YATES 1
1 Hematology Laboratory and the Hektoen Institute for Medical Research of
the Cook County Hospital, Chicago, Illinois.
1. A 33 week old Swiss female mouse that had been caged with AKR males
exhibited a "spontaneous" lymphoblastoma resembling the spontaneous disease
in the AKR strain.
2. Tumor cell suspensions of this animal did not grow when inoculated into
AKR mice.
3. Tumor cell suspensions grew progressively in both Swiss and DBA mice,
producing a leukemia.
4. Cell-free filtrates of leukemic Swiss or DBA mouse brain induced leukemia
in 48 of 106 adult Swiss mice inoculated intracerebrally and 39 of 55 animals
inoculated intraperitoneally. The leukemia appeared in the majority of the
animals within one to three weeks from the time of inoculation.
5. Cell-free filtrates of the leukemic tumor failed to induce leukemia under
the conditions of the experiment.
Submitted on December 11, 1956
Accepted on February 2, 1957
