Blood, 1957, Vol. 12, No. 7, pp. 644-648.
© 1957 American Society of Hematology, Inc.
Dithizone Staining of Myeloid Granules
WILLIAM F. MCNARY JR. 1
1 Department of Anatomy, Boston University Medical School.
A dithizone-acetone-water solution may be used to stain myeloid granules in
blood smears and bone marrow preparations. It is necessary to lake the erythrocyte before staining in order to remove interfering substances. The results indicate that zinc, known to be present its leukocytes, is cytoplasmic and not associated with the myeloid granules. It is suggested that the method described may
be of value to complement the peroxidase stain as it is rapid, simple, and utilizes
solutions which may be stored for lengthy periods.
Submitted on October 5, 1956
Accepted on January 27, 1957