Blood, 1957, Vol. 12, No. 6, pp. 549-558.
© 1957 American Society of Hematology, Inc.
The Combined Use of Typhoid Vaccine and P32 Labeling
to Assess Myelopoiesis
S. PERRY 1,
I. M. WEINSTEIN 1,
C. G. CRADDOCK JR. 1,
J. S. LAWRENCE 1,
GLORIA PAUL 1,
MARY H. BAKER 1,
JEANINE GREEN 1, and
GEORGE CIANKO 1
1 Department of Medicine, University of California Medical Center and the
Sawtelle Veterans Administration Center, Los Angeles, California.
1. The intravenous administration of killed typhoid organisms to dogs results
in the development of a severe leukopenia which is followed by a marked
leukocytosis.
2. The myelopoietic response of dogs to typhoid injections is quite similar to
the response following leukopheresis.
3. Leukocyte DNA-P32 studies indicate that in response to typhoid stimulation, as with leukopheresis, the bone marrow constitutes the main reservoir for
granulocytes contributing to peripheral blood leukocytosis.
4. Evidence is presented that, under these experimental conditions, leukocytes, after once having left the vascular tree, are unable to re-enter in any significant numbers.
5. The use of typhoid vaccine to stimulate leukocytosis combined with the use
of P32 to measure the fate of cells released from the marrow is presented as an
accurate and reasonably simple method to measure certain aspects of myelopoiesis
in the experimental animal.
Submitted on August 20, 1956
Accepted on November 3, 1956
