Blood, 1957, Vol. 12, No. 6, pp. 520-528.
© 1957 American Society of Hematology, Inc.
Studies on Thrombopoiesis
II. Thrombocytopoiesis in Vitro from the Bone Marrow of Patients
with Idiopathic Thrombocytopenic Purpura
G. IZAK 1,
D. NELKEN 1, and
MISS A. HERZOG 1
1 Department of Medicine/B, Hadassah University Hospital, and the Department of Clinical Microbiology, Hebrew University, Hadassah Medical School, Jerusalem,
Israel.
Thrombocytopoiesis was studied by direct, continuous observation in tissue
cultures of bone marrows taken from three patients with chronic idiopathic
thrombocytopenic purpura and from normals following the addition of a potent
anti-platelet serum to the culture media.
The process of platelet production was similar in these two conditions and
followed the pattern observed in bone marrow cultures of healthy individuals.
The breakdown of megakaryocytes of patients with idiopathic thrombocytopenic purpura and those treated with anti-platelet serum was greatly accelerated.
No morphologic evidence of injury to the megakaryocytes was present.
The platelets produced showed degenerative changes, they were agglutinated
and underwent phagocytosis by the myeloid elements and reticulum cells were
present in the cultures.
