Blood, 1957, Vol. 12, No. 6, pp. 507-519.
© 1957 American Society of Hematology, Inc.
Studies on Thrombopoiesis
I. Thrombocytopoiesis in Vitro: Experiments with Animal and
Normal Human Material
G. IZAK 1,
D. NELKEN 1,
J. GUREVITCH 1, and
MISS A. HERZOG 1
1 Departments of Medicine/B and Clinical Microbiology, Hadassah University
Hospital, Jerusalem, Israel.
Thrombocyte production from megakaryocytes of healthy humans, dogs,
guinea pigs and mice was observed continuously for one to six days in tissue
culture.
Approximately 70 per cent of the explanted megakaryocytes broke down to
give rise to numerous platelets, while the remaining 30 per cent of the cells
remained unchanged.
The newly formed thrombocytes were separated from the rest of the bone marrow tissue, counted and their serotonin absorbing capacity determined.
There was invariably a gradual increase in both the number of thrombocytes
and in their serotonin absorbing capacity during the one to six days of observation.
The results obtained were similar in human megakaryocytes and in those of
experimental animals.
