SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on March 27, 2003; DOI 10.1182/blood-2002-12-3834. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-12-3834v1 102/2/421    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Arber, C. Articles by Brown, J. M. Y. Search for Related Content PubMed PubMed Citation Articles by Arber, C. Articles by Brown, J. M. Y. Related Collections Immunobiology Transplantation Plenary Papers Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 July 2003, Vol. 102, No. 2, pp. 421-428 PLENARY PAPERS Common lymphoid progenitors rapidly engraft and protect against lethal murine cytomegalovirus infection after hematopoietic stem cell transplantation Caroline Arber, Andrew BitMansour, Timothy E. Sparer, John P. Higgins, Edward S. Mocarski, Irving L. Weissman, Judith A. Shizuru, and Janice M. Y. Brown From the Department of Medicine, Divisions of Bone Marrow Transplantation and Infectious Diseases; Departments of Pathology and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA. Lymphoid deficiency after allogeneic hematopoietic cell transplantation (HCT) results in increased susceptibility to infection; however, transplantation of mature lymphocytes frequently results in a serious complication known as graft-versus-host disease (GVHD). Here we demonstrate in mice that both congenic as well as allogeneic transplantation of low numbers of highly purified common lymphoid progenitors (CLPs)—a rare population of lymphoid-lineage-committed bone marrow cells—accelerates immune reconstitution after lethal irradiation and rescue with hematopoietic stem cells (HSCs). After congenic transplantation, 3 x 103 CLPs protected against murine cytomegalovirus (MCMV) infection at a level roughly equivalent to 107 unfractionated lymph node cells. In the allogeneic model of matched unrelated donor HSC transplantation, cotransplantation of 3 x 103 CLPs protected thymus-bearing as well as thymectomized hosts from MCMV infection and attenuated disease severity. Immunohistochemistry in combination with antibody depletion of T and natural killer (NK) cells confirmed that CLP-derived as well as residual host lymphocytes contribute to antiviral protection. Importantly, transplantation of allogeneic CLPs provided a durable antiviral immunity without inducing GVHD. These data support the potential for composing grafts with committed progenitors to reduce susceptibility to viral infection following HCT. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Jump-starting immune reconstitution after stem cell transplantation Kenneth I. Weinberg Blood 2003 102: 415. [Full Text] [PDF] This article has been cited by other articles: V. H. Nguyen, S. Shashidhar, D. S. Chang, L. Ho, N. Kambham, M. Bachmann, J. M. Brown, and R. S. Negrin The impact of regulatory T cells on T-cell immunity following hematopoietic cell transplantation Blood, January 15, 2008; 111(2): 945 - 953. [Abstract] [Full Text] [PDF] M. H. Dallas, B. Varnum-Finney, P. J. Martin, and I. D. Bernstein Enhanced T-cell reconstitution by hematopoietic progenitors expanded ex vivo using the Notch ligand Delta1 Blood, April 15, 2007; 109(8): 3579 - 3587. [Abstract] [Full Text] [PDF] E.-J. Wils, E. Braakman, G. M. G. M. Verjans, E. J. C. Rombouts, A. E. C. Broers, H. G. M. Niesters, G. Wagemaker, F. J. T. Staal, B. Lowenberg, H. Spits, et al. Flt3 Ligand Expands Lymphoid Progenitors Prior to Recovery of Thymopoiesis and Accelerates T Cell Reconstitution after Bone Marrow Transplantation J. Immunol., March 15, 2007; 178(6): 3551 - 3557. [Abstract] [Full Text] [PDF] I. Maillard, B. A. Schwarz, A. Sambandam, T. Fang, O. Shestova, L. Xu, A. Bhandoola, and W. S. Pear Notch-dependent T-lineage commitment occurs at extrathymic sites following bone marrow transplantation Blood, May 1, 2006; 107(9): 3511 - 3519. [Abstract] [Full Text] [PDF] M. E. Garcia-Ojeda, S. Dejbakhsh-Jones, D. Chatterjea-Matthes, A. Mukhopadhyay, A. BitMansour, I. L. Weissman, J. M. Y. Brown, and S. Strober Stepwise Development of Committed Progenitors in the Bone Marrow That Generate Functional T Cells in the Absence of the Thymus J. Immunol., October 1, 2005; 175(7): 4363 - 4373. [Abstract] [Full Text] [PDF] I. Weissman Stem Cell Research: Paths to Cancer Therapies and Regenerative Medicine JAMA, September 21, 2005; 294(11): 1359 - 1366. [Abstract] [Full Text] [PDF] R. Terra, I. Louis, R. Le Blanc, S. Ouellet, J. C. Zuniga-Pflucker, and C. Perreault T-cell generation by lymph node resident progenitor cells Blood, July 1, 2005; 106(1): 193 - 200. [Abstract] [Full Text] [PDF] A. F. List, J. Vardiman, J.-P. J. Issa, and T. M. DeWitte Myelodysplastic Syndromes Hematology, January 1, 2004; 2004(1): 297 - 317. [Abstract] [Full Text] [PDF] N. J. Chao, S. G. Emerson, and K. I. Weinberg Stem Cell Transplantation (Cord Blood Transplants) Hematology, January 1, 2004; 2004(1): 354 - 371. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020