SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on September 5, 2002; DOI 10.1182/blood-2002-06-1780. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-06-1780v1 101/2/383    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Akashi, K. Articles by Li, L. Search for Related Content PubMed PubMed Citation Articles by Akashi, K. Articles by Li, L. Related Collections Plenary Papers Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 January 2003, Vol. 101, No. 2, pp. 383-389 PLENARY PAPER Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis Koichi Akashi, Xi He, Jie Chen, Hiromi Iwasaki, Chao Niu, Brooke Steenhard, Jiwang Zhang, Jeff Haug, and Linheng Li From the Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA; Stowers Institute for Medical Research, Kansas City, MO; and Department of Mathematics and Statistics, University of Missouri, Kansas City. Hematopoietic stem cells (HSCs) maintain hematopoiesis by giving rise to all types of blood cells. Recent reports suggest that HSCs also possess the potential to generate nonhematopoietic tissues. To evaluate the underlying mechanisms in the commitment of HSCs into multitissue and multihematopoietic lineages, we performed oligonucleotide array analyses targeting for prospectively purified HSCs, multipotent progenitors (MPPs), common lymphoid progenitors (CLPs), and common myeloid progenitors (CMPs). Here we show that HSCs coexpress multiple nonhematopoietic genes as well as hematopoietic genes; MPPs coexpress myeloid and lymphoid genes; CMPs coexpress myeloerythroid, but not lymphoid genes, whereas CLPs coexpress T-, B-, and natural killer-lymphoid, but not myeloid, genes. Thus, the stepwise decrease in transcriptional accessibility for multilineage-affiliated genes may represent progressive restriction of developmental potentials in early hematopoiesis. These data support the hypothesis that stem cells possess a wide-open chromatin structure to maintain their multipotentiality, which is progressively quenched as they go down a particular pathway of differentiation. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Miranda-Saavedra, S. De, M. W. Trotter, S. A. Teichmann, and B. Gottgens BloodExpress: a database of gene expression in mouse haematopoiesis Nucleic Acids Res., January 1, 2009; 37(suppl_1): D873 - D879. [Abstract] [Full Text] [PDF] Y. Fukuchi, M. Ito, F. Shibata, T. Kitamura, and H. Nakajima Activation of CCAAT/Enhancer-Binding Protein {alpha} or PU.1 in Hematopoietic Stem Cells Leads to Their Reduced Self-Renewal and Proliferation Stem Cells, December 1, 2008; 26(12): 3172 - 3181. [Abstract] [Full Text] [PDF] I. G. Costa, S. Roepcke, C. Hafemeister, and A. Schliep Inferring differentiation pathways from gene expression Bioinformatics, July 1, 2008; 24(13): i156 - i164. [Abstract] [Full Text] [PDF] B. S. Braun and K. Shannon Targeting Ras in Myeloid Leukemias Clin. Cancer Res., April 15, 2008; 14(8): 2249 - 2252. [Abstract] [Full Text] [PDF] S. Luc, K. Anderson, S. Kharazi, N. Buza-Vidas, C. Boiers, C. T. Jensen, Z. Ma, L. Wittmann, and S. E. W. Jacobsen Down-regulation of Mpl marks the transition to lymphoid-primed multipotent progenitors with gradual loss of granulocyte-monocyte potential Blood, April 1, 2008; 111(7): 3424 - 3434. [Abstract] [Full Text] [PDF] A. Rosa, M. Ballarino, A. Sorrentino, O. Sthandier, F. G. De Angelis, M. Marchioni, B. Masella, A. Guarini, A. Fatica, C. Peschle, et al. The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation PNAS, December 11, 2007; 104(50): 19849 - 19854. [Abstract] [Full Text] [PDF] S. M. Graham, J. K. Vass, T. L. Holyoake, and G. J. Graham Transcriptional Analysis of Quiescent and Proliferating CD34+ Human Hemopoietic Cells from Normal and Chronic Myeloid Leukemia Sources Stem Cells, December 1, 2007; 25(12): 3111 - 3120. [Abstract] [Full Text] [PDF] I. Glauche, M. Cross, M. Loeffler, and I. Roeder Lineage Specification of Hematopoietic Stem Cells: Mathematical Modeling and Biological Implications Stem Cells, July 1, 2007; 25(7): 1791 - 1799. [Abstract] [Full Text] [PDF] R. S. Welner, R. Pelayo, K. P. Garrett, X. Chen, S. S. Perry, X.-H. Sun, B. L. Kee, and P. W. Kincade Interferon-producing killer dendritic cells (IKDCs) arise via a unique differentiation pathway from primitive c-kitHiCD62L+ lymphoid progenitors Blood, June 1, 2007; 109(11): 4825 - 4931. [Abstract] [Full Text] [PDF] D. J. Greenway, M. Street, A. Jeffries, and N. J. Buckley RE1 Silencing Transcription Factor Maintains a Repressive Chromatin Environment in Embryonic Hippocampal Neural Stem Cells Stem Cells, February 1, 2007; 25(2): 354 - 363. [Abstract] [Full Text] [PDF] V. Jankovic, A. Ciarrocchi, P. Boccuni, T. DeBlasio, R. Benezra, and S. D. Nimer Id1 restrains myeloid commitment, maintaining the self-renewal capacity of hematopoietic stem cells PNAS, January 23, 2007; 104(4): 1260 - 1265. [Abstract] [Full Text] [PDF] S. R. L. Young, C. Mumaw, J. A. Marrs, and D. G. Skalnik Antisense Targeting of CXXC Finger Protein 1 Inhibits Genomic Cytosine Methylation and Primitive Hematopoiesis in Zebrafish J. Biol. Chem., December 1, 2006; 281(48): 37034 - 37044. [Abstract] [Full Text] [PDF] L. Warren, D. Bryder, I. L. Weissman, and S. R. Quake Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR PNAS, November 21, 2006; 103(47): 17807 - 17812. [Abstract] [Full Text] [PDF] E. Chapiro, E. Delabesse, V. Asnafi, C. Millien, F. Davi, E. Nugent, K. Beldjord, T. Haferlach, D. Grimwade, and E. A. Macintyre Expression of T-lineage-affiliated transcripts and TCR rearrangements in acute promyelocytic leukemia: implications for the cellular target of t(15;17) Blood, November 15, 2006; 108(10): 3484 - 3493. [Abstract] [Full Text] [PDF] C Massard, E Deutsch, and J-C Soria Tumour stem cell-targeted treatment: elimination or differentiation Ann. Onc., November 1, 2006; 17(11): 1620 - 1624. [Abstract] [Full Text] [PDF] E. M. Bruscia, E. C. Ziegler, J. E. Price, S. Weiner, M. E. Egan, and D. S. Krause Engraftment of Donor-Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice Stem Cells, October 1, 2006; 24(10): 2299 - 2308. [Abstract] [Full Text] [PDF] A. Y. Lai and M. Kondo Asymmetrical lymphoid and myeloid lineage commitment in multipotent hematopoietic progenitors J. Exp. Med., August 7, 2006; 203(8): 1867 - 1873. [Abstract] [Full Text] [PDF] D. Bryder, D. J. Rossi, and I. L. Weissman Hematopoietic Stem Cells: The Paradigmatic Tissue-Specific Stem Cell Am. J. Pathol., August 1, 2006; 169(2): 338 - 346. [Abstract] [Full Text] [PDF] A. Huttmann, U. Duhrsen, K. Heydarian, L. Klein-Hitpass, T. Boes, A. W. Boyd, and C.-L. Li Gene Expression Profiles in Murine Hematopoietic Stem Cells Revisited: Analysis of cDNA Libraries Reveals High Levels of Translational and Metabolic Activities Stem Cells, July 1, 2006; 24(7): 1719 - 1727. [Abstract] [Full Text] [PDF] G. O. Gillard and A. G. Farr Features of Medullary Thymic Epithelium Implicate Postnatal Development in Maintaining Epithelial Heterogeneity and Tissue-Restricted Antigen Expression J. Immunol., May 15, 2006; 176(10): 5815 - 5824. [Abstract] [Full Text] [PDF] K. Inoue, N. Ogonuki, H. Miki, M. Hirose, S. Noda, J.-M. Kim, F. Aoki, H. Miyoshi, and A. Ogura Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer J. Cell Sci., May 15, 2006; 119(10): 1985 - 1991. [Abstract] [Full Text] [PDF] L. M. Kamminga and G. de Haan Cellular Memory and Hematopoietic Stem Cell Aging Stem Cells, May 1, 2006; 24(5): 1143 - 1149. [Abstract] [Full Text] [PDF] T. Jaatinen, H. Hemmoranta, S. Hautaniemi, J. Niemi, D. Nicorici, J. Laine, O. Yli-Harja, and J. Partanen Global Gene Expression Profile of Human Cord Blood-Derived CD133+ Cells Stem Cells, March 1, 2006; 24(3): 631 - 641. [Abstract] [Full Text] [PDF] T. V. Bowman, A. J. McCooey, A. A. Merchant, C. A. Ramos, P. Fonseca, A. Poindexter, S. B. Bradfute, D. M. Oliveira, R. Green, Y. Zheng, et al. Differential mRNA Processing in Hematopoietic Stem Cells Stem Cells, March 1, 2006; 24(3): 662 - 670. [Abstract] [Full Text] [PDF] C. J. Luckey, D. Bhattacharya, A. W. Goldrath, I. L. Weissman, C. Benoist, and D. Mathis Memory T and memory B cells share a transcriptional program of self-renewal with long-term hematopoietic stem cells PNAS, February 28, 2006; 103(9): 3304 - 3309. [Abstract] [Full Text] [PDF] N. Onai, A. Obata-Onai, R. Tussiwand, A. Lanzavecchia, and M. G. Manz Activation of the Flt3 signal transduction cascade rescues and enhances type I interferon-producing and dendritic cell development J. Exp. Med., January 23, 2006; 203(1): 227 - 238. [Abstract] [Full Text] [PDF] J. Lotem, D. Netanely, E. Domany, and L. Sachs Human cancers overexpress genes that are specific to a variety of normal human tissues PNAS, December 20, 2005; 102(51): 18556 - 18561. [Abstract] [Full Text] [PDF] D. C. Taussig, D. J. Pearce, C. Simpson, A. Z. Rohatiner, T. A. Lister, G. Kelly, J. L. Luongo, G.-a. H. Danet-Desnoyers, and D. Bonnet Hematopoietic stem cells express multiple myeloid markers: implications for the origin and targeted therapy of acute myeloid leukemia Blood, December 15, 2005; 106(13): 4086 - 4092. [Abstract] [Full Text] [PDF] C. K. Taranger, A. Noer, A. L. Sorensen, A.-M. Hakelien, A. C. Boquest, and P. Collas Induction of Dedifferentiation, Genomewide Transcriptional Programming, and Epigenetic Reprogramming by Extracts of Carcinoma and Embryonic Stem Cells Mol. Biol. Cell, December 1, 2005; 16(12): 5719 - 5735. [Abstract] [Full Text] [PDF] S. X. Liang, R. Summer, X. Sun, and A. Fine Gene expression profiling and localization of Hoechst-effluxing CD45- and CD45+ cells in the embryonic mouse lung Physiol Genomics, October 17, 2005; 23(2): 172 - 181. [Abstract] [Full Text] [PDF] A. Y. Lai, S. M. Lin, and M. Kondo Heterogeneity of Flt3-Expressing Multipotent Progenitors in Mouse Bone Marrow J. Immunol., October 15, 2005; 175(8): 5016 - 5023. [Abstract] [Full Text] [PDF] J.-R. Landry, S. Kinston, K. Knezevic, I. J. Donaldson, A. R. Green, and B. Gottgens Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cells Blood, October 15, 2005; 106(8): 2680 - 2687. [Abstract] [Full Text] [PDF] K. Bartolovic, S. Balabanov, B. Berner, H.-J. Buhring, M. Komor, S. Becker, D. Hoelzer, L. Kanz, W.-K. Hofmann, and T. H. Brummendorf Clonal Heterogeneity in Growth Kinetics of CD34+CD38- Human Cord Blood Cells In Vitro Is Correlated with Gene Expression Pattern and Telomere Length Stem Cells, August 1, 2005; 23(7): 946 - 957. [Abstract] [Full Text] [PDF] G. O. Gillard and A. G. Farr Contrasting models of promiscuous gene expression by thymic epithelium J. Exp. Med., July 5, 2005; 202(1): 15 - 19. [Abstract] [Full Text] [PDF] D. J. Rossi, D. Bryder, J. M. Zahn, H. Ahlenius, R. Sonu, A. J. Wagers, and I. L. Weissman Cell intrinsic alterations underlie hematopoietic stem cell aging PNAS, June 28, 2005; 102(26): 9194 - 9199. [Abstract] [Full Text] [PDF] T. M. Schlaeger, H. K. A. Mikkola, C. Gekas, H. B. Helgadottir, and S. H. Orkin Tie2Cre-mediated gene ablation defines the stem-cell leukemia gene (SCL/tal1)-dependent window during hematopoietic stem-cell development Blood, May 15, 2005; 105(10): 3871 - 3874. [Abstract] [Full Text] [PDF] J. Schwable, C. Choudhary, C. Thiede, L. Tickenbrock, B. Sargin, C. Steur, M. Rehage, A. Rudat, C. Brandts, W. E. Berdel, et al. RGS2 is an important target gene of Flt3-ITD mutations in AML and functions in myeloid differentiation and leukemic transformation Blood, March 1, 2005; 105(5): 2107 - 2114. [Abstract] [Full Text] [PDF] J. T. Opferman, H. Iwasaki, C. C. Ong, H. Suh, S.-i. Mizuno, K. Akashi, and S. J. Korsmeyer Obligate Role of Anti-Apoptotic MCL-1 in the Survival of Hematopoietic Stem Cells Science, February 18, 2005; 307(5712): 1101 - 1104. [Abstract] [Full Text] [PDF] K. Theilgaard-Monch, L. C. Jacobsen, R. Borup, T. Rasmussen, M. D. Bjerregaard, F. C. Nielsen, J. B. Cowland, and N. Borregaard The transcriptional program of terminal granulocytic differentiation Blood, February 15, 2005; 105(4): 1785 - 1796. [Abstract] [Full Text] [PDF] R. Haddad, P. Guardiola, B. Izac, C. Thibault, J. Radich, A.-L. Delezoide, C. Baillou, F. M. Lemoine, J. C. Gluckman, F. Pflumio, et al. Molecular characterization of early human T/NK and B-lymphoid progenitor cells in umbilical cord blood Blood, December 15, 2004; 104(13): 3918 - 3926. [Abstract] [Full Text] [PDF]