|
|
Prepublished online as a Blood First Edition Paper on August 22, 2002; DOI 10.1182/blood-2002-02-0486.
 Previous Article | Table of Contents | Next Article 
Blood, 1 January 2003, Vol. 101, No. 1, pp. 45-51
CHEMOKINES
Heterologous cells cooperate to augment stem cell migration,
homing, and engraftment
Gregor B. Adams,
Karissa T. Chabner,
Russell B. Foxall,
Kathryn W. Weibrecht,
Neil P. Rodrigues,
David Dombkowski,
Robert Fallon,
Mark C. Poznansky, and
David T. Scadden
From the Partners AIDS Research Center and MGH Cancer
Center, Harvard Medical School, Massachusetts General Hospital, Boston;
and Indiana University Cancer Research Institute, Indiana University
School of Medicine, Indianapolis.
T-lymphocyte depletion of bone marrow grafts compromises
engraftment, suggesting a facilitating mechanism provided by the T
cells that has been shown to associate with CD8+ but not
CD4+ T cells. Explanations for this phenomenon have focused
on immune targeting of residual host cells or cytokine production. We
provide evidence for an alternative mechanism based on cooperative
effects on cell motility. We observed that engraftment of
CD34+ cells in a  2-microglobulin-deficient
nonobese diabetic/severe combined immunodeficiency
(2m / NOD/SCID) mouse model paralleled
clinical observations in humans, with an enhancing effect noted from
the addition of CD8+ cells but not CD4+ cells.
This correlated with CD8+ augmentation of CD34+
cell homing to the bone marrow in vivo and CD8+
cell-associated increases of CD34+ cell transmigration
through a bone marrow endothelial cell line in vitro. The cooperative
interaction was not sensitive to brefeldin A inhibition of protein
secretion. However, cytochalasin D-induced inhibition of
CD8+ cytoskeletal rearrangements abrogated
CD34+ transendothelial migration and impaired
CD34+ cell homing in vivo. CD8+ cells did not
migrate in tandem with CD34+ cells or alter endothelial
barrier integrity; rather, they affected phosphotyrosine-mediated
signaling in CD34+ cells in response to the chemokine
stromal derived factor-1 (SDF-1). These data demonstrate
cell-cell cooperativity between different cell types in mediating
chemotactic events and provide one potential explanation for the
clinically observed effect of CD8+ cells on bone marrow
transplantation. This modification of cell migration by neighboring
cells provides broad possibilities for combinatorial effects between
cells of different types to influence cell localization.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Bridenbaugh, L. Kenins, E. Bouliong-Pillai, C. P. Kalberer, E. Shklovskaya, A. Gratwohl, and A. Wodnar-Filipowicz
Clinical stem-cell sources contain CD8+CD3+ T-cell receptor-negative cells that facilitate bone marrow repopulation with hematopoietic stem cells
Blood,
February 1, 2008;
111(3):
1735 - 1738.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Li, H. Hisha, R. Yasumizu, T.-X. Fan, G.-X. Yang, Q. Li, Y.-Z. Cui, X.-L. Wang, C.-Y. Song, S. Okazaki, et al.
Analyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes
Stem Cells,
May 1, 2007;
25(5):
1186 - 1194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. H. Peranteau, M. Endo, O. O. Adibe, A. Merchant, P. W. Zoltick, and A. W. Flake
CD26 inhibition enhances allogeneic donor-cell homing and engraftment after in utero hematopoietic-cell transplantation
Blood,
December 15, 2006;
108(13):
4268 - 4274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Lan, N. Tonomura, A. Shimizu, S. Wang, and Y.-G. Yang
Reconstitution of a functional human immune system in immunodeficient mice through combined human fetal thymus/liver and CD34+ cell transplantation
Blood,
July 15, 2006;
108(2):
487 - 492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Abbasian, D. Mahmud, N. Mahmud, S. Chunduri, H. Araki, P. Reddy, R. Hoffman, M. Arpinati, J. L. M. Ferrara, and D. Rondelli
Allogeneic T cells induce rapid CD34+ cell differentiation into CD11c+CD86+ cells with direct and indirect antigen-presenting function
Blood,
July 1, 2006;
108(1):
203 - 208.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Lapidot, A. Dar, and O. Kollet
How do stem cells find their way home?
Blood,
September 15, 2005;
106(6):
1901 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. B. Slayton, D. A. Wainman, X. M. Li, Z. Hu, A. Jotwani, C. R. Cogle, D. Walker, R. C. Fisher, J. R. Wingard, E. W. Scott, et al.
Developmental Differences in Megakaryocyte Maturation Are Determined by the Microenvironment
Stem Cells,
September 1, 2005;
23(9):
1400 - 1408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Urbich and S. Dimmeler
Endothelial Progenitor Cells: Characterization and Role in Vascular Biology
Circ. Res.,
August 20, 2004;
95(4):
343 - 353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nakamura, M. Inaba, K. Sugiura, T. Yoshimura, A-H. Kwon, Y. Kamiyama, and S. Ikehara
Enhancement of Allogeneic Hematopoietic Stem Cell Engraftment and Prevention of GvHD by Intra-Bone Marrow Bone Marrow Transplantation Plus Donor Lymphocyte Infusion
Stem Cells,
March 1, 2004;
22(2):
125 - 134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-C. Lee, T. Cheng, G. B. Adams, E. C. Attar, N. Miura, S. B. Lee, Y. Saito, I. Olszak, D. Dombkowski, D. P. Olson, et al.
P2Y-like receptor, GPR105 (P2Y14), identifies and mediates chemotaxis of bone-marrowhematopoietic stem cells
Genes & Dev.,
July 1, 2003;
17(13):
1592 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
