Blood, 1955, Vol. 10, No. 5, pp. 453-457.
© 1955 American Society of Hematology, Inc.
Absorption Microspectroscopy of Bone Marrow Cells
SÉRGIO DE CARVALHO 1
1 Institute of Histology and Embryology, Faculty of Medicine, Lisbon, Portugal.
Using a microspectrographic technic, reflecting optics and narrow bands of the
continuum (5500, 4150 and 2650 Å), the author has obtained pictures of human
and rat bone marrow cells in unfixed dry smears as well as in suspensions in
vivo; erythroblasts showed different absorption patterns. Violet light microspectrography, specific for heme absorption, allowed us to demonstrate the presence
of intranuclear heme granules through various stages of maturation of these
cells. This confirmed previous and simultaneous results arrived at by cytochemical and other methods.
The presence of nucleic acids has been demonstrated in neutrophil granulations.
Submitted on August 17, 1954
Accepted on October 31, 1954
